Isolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction
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Isolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction
Procedures and conditions have been established such that the unstable enzyme-bound flavin intermediate produced in the bacterial luciferase reacti~ can be isolated as approximately 70% of the flavin product, the remaining being the final product, FMN. The structure of the intermediate is proposed to be that of a luciferase-bound 4a,5-dihydroflavin-4a-hydroxide. The intermediate has a half-life...
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The reaction of luciferase-bound Z is known to result in the formation of a long-lived intermedlate in the bioluminescent reaction (1). This intermediate was isolated and ,haracterized as the luciferase-peroxyflavin (2), whose struc~ture was later shown to be the flavin 4a-substituted peroxyadduct (3). In the earlier work this peroxy intermediate had been shown to exhibit a single peak at about...
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The identity and stoichiometry of the reaction products of the oxygenated reduced flavin bacterial luciferase intermediate isolated by Sephadex chromatography at low temperature have been determined under two conditions, allowing the reaction to go to completion by warming either in the presence or absence of long chain aliphatic aldehyde. In the latter case, very little bioluminescence occurs,...
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Bioluminescence catalyzed by bacterial luciferases was measured using FMN, iso-FMN (6methyl-8-nor-FMN), and FMN analogs carrying the following substituents a t position 8: -H, -C1, -F, S M e , S O M e , S 0 2 M e , or -0Me. The first-order rate constants for the decay of light emission correlate with the one-electron oxidation potentials of the 4a,5-dihydro forms of the FMN analogs. To determin...
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Bacterial luciferase catalyzes the emission of visible light from the reaction of reduced flavin, molecular oxygen, and an n-alkyl aldehyde. The mechanism of the reaction was probed by measuring the electronic effects of various substituents at the 8-position of the flavin ring system. Substituent effects were obtained for CH3-, Cl-, CH3O-, CH3S-, F-, and H- on the rate of formation and decay o...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - General Subjects
سال: 1987
ISSN: 0304-4165
DOI: 10.1016/0304-4165(87)90076-6